Direct wine analysis was used to determine the molecular weight of the components and their variable content across the 5 samples in this study was observed. This work demonstrates the utility of analyzing complex mixtures without prior sample preparation by making use of the separation of HPLC combined with the High Resolution Accurate Mass (HRAM) of the Q Exactive quadrupole Orbitrap mass analyzer. The combination of these techniques provides a robust and confident means of profiling complex mixtures as well as successful identification of detected components.

Flavonoids, a class of polyphenolic compounds found in tea, plants and many fruits, have shown some promising results related to anti-cancer as well as anti-inflammatory and anti-allergy properties. For these reasons, there has been renewed interest in both detecting and characterizing these compounds. We demonstrate the utility of MSn for structural determination and unambiguous classification of a suspected flavonoid compound and unambiguous determination of product origin.

Red wine is a very complex mixture and a rich source of beneficial anti-oxidants. Identification and quantitation of these natural products is challenging. Ultra High Pressure Liquid Chromatography (U-HPLC) coupled to the Thermo Scientific LTQ Orbitrap XL mass spectrometer was used for analysis of French red wine, which enabled simultaneous detection and relative quantitation of the wine's anti-oxidant constituents.

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of catechins. The separation is performed on six different stationary phases; Thermo Scientific Acclaim C30, Acclaim Mixed-Mode HILIC-1, Acclaim Phenyl-1, Acclaim 120 C18, Acclaim PolarAdvanage and Acclaim PolarAdvantage II. The results were compared.

The Thermo Scientific Ultimate 3000 RS system is applied for the assay analysis of Rutoside Trihydrate. The Thermo Scientific Accucore aQ column separated the rutoside trihydrate from the impurities present in a solid dosage formulation in under 15 minutes.