LC-MS/MS Analysis of EtG and EtS in Dilute Urine on the TSQ Endura Triple Quadrupole MS

Instrument Type: LCMSMS

We developed a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for the forensic toxicological analysis of EtG and EtS in urine with limits of quantitation (LOQs) of 100 and 50 ng/mL, respectively, using only urine dilution as sample preparation. For forensic use only.

CAN101: Detection of Ethyl Glucuronide Using Pulsed Electrochemical Detection following Reversed Phase High Performance Liquid Chromatography

Instrument Type: IC

This application note discusses improvements made to the previous method. One change involves mobile phase optimization, in which alternative organic modifiers are studied. Another change is the addition of a wash step, which prevents carryover of the sample matrix between runs. Adjustments made to the SPE provide a much higher sample recovery. The internal standard is also changed to ProG in order to prevent elution with matrix interferents.