AU72197 : Determination of Tobramycin Using HPAE-IPAD on a Compact IC System: 20 µM tobramycin

Instrument Type: IC

Tobramycin is an important aminoglycoside antibiotic used in ophthalmic and intravenous treatments to treat bacterial infections by blocking protein synthesis.[1] Determinations of tobramycin, kanamycin and other aminoglycoside antibiotics have been previously demonstrated by HPAE-IPAD since 2004.[2–6] This document updates Thermo Scientific™ Application Note 61 using a highpressure-capable Dionex Integrion HPIC system equipped with updated EG technology and an electrochemical detector.

Determination of High Sugar Concentrations in a Basalmic Vinegar Sample Using a Compact Ion Chromatography System

Instrument Type: IC

This record demonstrates the determinations of glucose and fructose in a 100-fold diluted basalmic vinegar sample by HPAE-PAD. Typically samples with g/L concentrations require >10,000-fold dilutions to remain in the linear range of the very sensitive HPAE-PAD technique. However, this method demonstrated on the Integrion RFIC system, uses a 0.4 µL internal injection loop with the High Concentration Carbohydrate Analysis Kit to extend the linearity from low mg/L to g/L concentrations. A wood hydrolysate application using this technique is attached for reference.

Determination of High Sugar Concentrations in a Scotch Liqueur Sample Using a Compact Ion Chromatography System

Instrument Type: IC

This record demonstrates the determinations of glucose, fructose, and sucrose in a 100-fold diluted liqueur alcoholic beverage sample by HPAE-PAD. Typically samples with g/L concentrations require >10,000-fold dilutions to remain in the linear range of the very sensitive HPAE-PAD technique. However, this method demonstrated on the Integrion RFIC system, uses a 0.4 µL internal injection loop with the High Concentration Carbohydrate Analysis Kit to extend the linearity from low mg/L to g/L concentrations. A wood hydrolysate application using this technique is attached for reference.