In this application, three detectors were employed and compared. An IonPac CS18 column coupled to IPAD to detect biogenic amines in beer and wine samples prior to storage. As relatively little information exists on the accumulation of biogenic amines in alcoholic beverages during storage, refrigerated samples were analyzed using suppressed conductivity detection coupled to IPAD. UV detection was used to confirm the presence of tyramine in some alcoholic beverages. Detectors were also compared in terms of linearity, detection limits, precision, and recovery of biogenic amines spiked in samples.

The IonPac CS18, a polymeric weak acid cationexchange column, was used to separate biogenic amines in a variety of fermented and non-fermented food samples, with detection by IPAD, suppressed conductivity, and UV. The described method uses a simple electrolytically generated MSA eluent without requiring the use of solvents or aggressive eluent systems that have previously been reported. In addition, the method results in good precision and recovery over a wide range of sample matrices and avoids the need for complex and long derivatization procedures.

The Thermo Scientific HPLC system is applied for the analysis of catecholamines and serotonin at physiological concentrations. The separation is performed on a Thermo Scientific Acclaim 120 C18 column, with electrochemical detection.

The Thermo Scientific HPLC system is applied for the analysis of catecholamines. The separation is performed on a Thermo Scientific Hypersil GOLD aQ HPLC column, with UV detection at 270 nm.

The Thermo Scientific HPLC system is applied for the determination of catecholamines. The separation is performed on a Thermo Scientific Syncronis HILIC HPLC column, with UV detection at 280 nm.