The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of trimethoprim and its impurities. The separation was performed on a Thermo Scientific Acclaim 120 C18 HPLC column using the method described in the USP 38 monograph. The results obtained for resolution exceeded the criteria stated in the USP. Resolution between trimethoprim and diaveridine peak was 2.97 (USP criteria of not less than 2.5).

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of trimethoprim and its impurities (H and I). The separation was performed on a Thermo Scientific Betasil CN HPLC column using the method described in the EP 8.0 monograph. The results obtained for resolution and relative retention time (RRT) exceeded the criteria stated in the EP. Resolution between impurity B and trimethoprim was 5.9 (EP criteria of not less than 2.0), RRT of impurity H was 1.8 (EP criteria of about 1.8).

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of trimethoprim and its impurities (E, D, G, B, J and F). The separation was performed on a Thermo Scientific Hypersil Gold HPLC column using the method described in the EP 8.0 monograph. The results obtained for resolution and tailing factor exceeded the criteria stated in the EP. Resolution between impurity E and trimethoprim was 7.7 (EP criteria of not less than 2.5) and tailing factor for trimethoprim was 1.1 (EP criteria not more than 2.0).

The Thermo Scientific UltiMate 3000 HPLC system is applied for the determination of doxorubicin in human serum. The samples are prepared using Thermo Scientific SOLA solid phase extraction (SPE) cartridges, and the separation performed on a Thermo Scientific Hypersil GOLD HPLC column, with fluorescence detection. For Research Use Only. Not for use in diagnostic procedures.

The Thermo Scientific Dionex UltiMate 3000 HPLC system is applied for the analysis of vinorelbine and its impurities. The separation was performed on a Thermo Scientific Hypersil GOLD 5µm column with UV detection at 267 nm.