This work develops methods for the determination of carbohydrates in wood acid hydrolysates using a CarboPac SA10-4μm column, along with a 0.4 µL injection and thicker working electrode gasket. This column contains smaller resin particles, enabling high-resolution, high-efficiency separation, particularly for the rhamnose-galactose that has been challenging to resolve with previous methods. The described method is fast, provides good sensitivity and consistent response, and can be routinely used to determine sugars in wood samples. An ICS-6000 can be used for this application.

This work develops methods for the determination of carbohydrates in wood acid hydrolysates using the CarboPac SA10-4μm column, along with a 0.4 µL injection and thicker working electrode gasket. This column contains smaller resin particles, enabling high-resolution, high-efficiency separation, particularly for rhamnose-galactose that has been challenging to resolve with previous methods. The described method is fast, provides good sensitivity and consistent response. An ICS-6000 can be used for this application.

This work updates the column used in AN1161 with a shorter 4 × 150 mm format column. The combination of the smaller resin particle size of the Dionex CarboPac SA10-4μm column and the shorter column used here results in separation of eight common sugars in less than six minutes. This is significant time savings as compared to the eight-minute runtime achieved in AN1089. The shorter run time allows for faster sample turnaround times and reduced eluent consumption, thereby improving the overall process economics. Results for method linearity, accuracy, and robustness are presented here.

The current work updates the system used for the carbohydrate analysis in AN1089. The new system combines flexibility and ease-of-use with high sensitivity and selectivity, bringing a higher level of convenience and cost effectiveness to simple sugar analysis. The method proposed here separates eight common carbohydrate sugars in less than eight minutes. The shorter run time results in better process economics. Using this method, carbohydrates present in 10 individual biofuel samples were quantified.

The current study updates the fast method described in AN280 with a Dionex CarboPac SA10-4 μm column. Due to the smaller particle size, this column provides more efficient peaks and better resolution, making the sample analysis easier and more reliable. Moreover, the system used for the analysis is updated to the Thermo Scientific™ Dionex™ Integrion™ HPIC™ system. This system combines flexibility and ease-of-use with high sensitivity and selectivity, bringing a new level of convenience and cost effectiveness to simple sugar analysis.