AN335: Accelerated Solvent Extraction (ASE) of Active Ingredients from Natural Products

Instrument Type: HPLC

Herbal products, nutraceuticals, and other food supplements are subject to increasingly rigorous investigation and development. Industry standardization, as well as labeling requirements under new federal regulations, have prompted tighter regulation and monitoring of the active ingredients in these products . This Application Note describes the use of ASE for extraction of commercially available nutritional supplements, specifically hypericin from Hypericum perforatum (St. John's wort) and berberine from Hydrastis canadenis (goldenseal root).

An improved analysis of alkaloids in goldenseal root on a Thermo Scientific Acclaim 120 C18 HPLC column with Thermo Scientific Dionex ASE Accelerated Solvent Extractor

Instrument Type: HPLC

The alkaloids present in goldenseal root were extracted using a Thermo Scientific Dionex ASE Accelerated Solvent Extractor system before separation by HPLC with UV detection. The separation of hydrastine and berberine was performed in less than 10 minutes on a Thermo Scientific Acclaim 120 C18 HPLC column.