Simultaneous Analysis of Amino Acids, Acylcarnitines, and Succinylacetone in Dried Blood Spots for Research Using Nonderivatized and Derivatized Methods

Instrument Type: LCMSMS

We developed a flow injection analysis-tandem mass spectrometry (FIA-MS/MS) research methods for simultaneous detection and quantification of 12 amino acids, 18 acylcarnitines, and succinylacetone in dried blood spots and compare derivatization and nonderivatization sample preparation methods.

Label free analysis of amino acids using UHPLC with charged aerosol detection

Instrument Type: UHPLC

Amino acids present in protein formulations serve as buffers, bulking agents, stabilizers, and antioxidants. Eight specific amino acids are commonly used as excipients in protein therapeutic formulations due to their physicochemical properties. These include Arginine, Aspartic acid, Glutamic acid, Lysine, Proline, Glycine, Histidine, and Methionine. The UHPLC column, an Acclaim PA2 column, provides excellent separation characteristics for these un-labeled amino acids. Gradient conditions are adjusted by selecting appropriate ion-pairing reagents, pH and level of organic solvents.

AN150: Determination of Amino Acids in Cell Cultures and Fermentation Broths.

Instrument Type: IC

This application note describes the use of AAA-Direct to analyze common amino acids in the presence of simple sugars, sugar alcohols, alcohols, and glycols in yeast and bacterial fermentation broths. In this application note, the AminoPac PA10 anion-exchange column is used to separate amino acid and carbohydrate ingredients in cell culture and fermentation broth media. High carbohydrate concentrations can hamper the determination of some amino acids due to coelution. Also in this application note, disposable gold (Au) electrodes are evaluated for use in amino acid determination.

Simple HILIC separation of arginine and glutamic acid without derivatization using HPLC-UV

Instrument Type: HPLC

The Thermo Scientific Dionex Ultimate 3000 system is applied for the analysis of two amino acids. The separation or arginine and glutamic acid was performed in less than 10 minutes on a Thermo Scientific Acclaim Mixed-Mode HILIC-1 HPLC column without the need for derivatization.