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Displaying 1-2 of 2 results for Tag: Acclaim RSLC C18

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Comparison of two reversed-phase columns for the analysis of tryptic digests of proteins

Instrument Type: HPLC

The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of tryptic digests of proteins. The separation is performed on Thermo Scientific Acclaim RSLC C18 and Acclaim RSLC PolarAdvantage II 2.2µm columns, with UV detection at 210 nm.

Increased resolution of tryptic digests of proteins using a Thermo Scientific Acclaim RSLC C18 HPLC column

Instrument Type: UHPLC

In this work, a digestion of bovine serum albumin is resolved using the Thermo Scientific Acclaim RSLC C18 into 127 peaks with an average peak width of 0.27 minutes. For a 90 minute gradient, this works out to a theoretical peak capacity of >300.