TN148: Automated Extraction and Determination of Human Hormones in Drinking Water Using Solid-Phase Extraction and HPLC with UV Detection

Instrument Type: HPLC

This Technical Note describes the use of high-surface-area solid phase extraction (SPE) to extract seven human hormones from drinking water using the Dionex AutoTrace 280 SPE instrument. Extracts were subsequently quantified using high-performance liquid chromatography (HPLC) to determine the recovery.

An improved analysis of multiple NITC derivatives of volatile amines using Thermo Scientific Acclaim RSLC 120 C18 HPLC column

Instrument Type: UHPLC

This work demonstrates the analysis of the multiple NITC derivatives of volatine amines using the Thermo Scientific Acclaim RSLC 120 C18 column with reversed-phase, gradient elution. 1-naphthyl isothiocyanate (NITC) reacts with amines in the trap, to produce a UV-absorbing thiourea derivative. This approach provides improved peak shape, better detection limits, faster analysis time and overall better economy.