AU72197 : Determination of Tobramycin Using HPAE-IPAD on a Compact IC System: 20 µM tobramycin

Instrument Type: IC

Tobramycin is an important aminoglycoside antibiotic used in ophthalmic and intravenous treatments to treat bacterial infections by blocking protein synthesis.[1] Determinations of tobramycin, kanamycin and other aminoglycoside antibiotics have been previously demonstrated by HPAE-IPAD since 2004.[2–6] This document updates Thermo Scientific™ Application Note 61 using a highpressure-capable Dionex Integrion HPIC system equipped with updated EG technology and an electrochemical detector.

TN50: Determination of the Amino Acid Content of Peptides by AAA-Direct

Instrument Type: IC

This Technical Note describes the peptide hydrolysis procedures that lead to successful determinations of amino acid composition using AAA-Direct. Results for the amino analysis of two peptides, Melanocyte Stimulating Hormone (MSH) and Luteinizing Hormone-Releasing Hormone (LH-RH), using both hydrochloric acid and methanesulfonic acid (MSA) as hydrolyzing reagents are presented to demonstrate the performance of this method.