This application note demonstrates that spectinomycin dihydrochloride and related impurities can be separated with a Dionex IonPac AmG-3µm C18 column using a simple eluent method (0.1M TFA) compared to the method in the EP monograph. The separation, repeatability, and sensitivity of this method were found to meet or exceed the current EP Spectinomycin Dihydrochloride monograph performance requirements. This method is reliable and can be used for the routine monitoring of spectinomycin.

The Thermo Scientific HPLC system is applied for the determination of spectinomycin and lincomycin. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage (PA) HPLC column, with electrochemical detection.