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HPLC HPLC (Biocompatible) UHPLC IC LCMS LCMSMS Automated Sample Prep

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BioPharma

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0.05%TFA 3% MeCN in water Acetonitrile Bovine serum albumin digest Buffer Digest Drug product Lysozyme digest mAb standards N/A NA Ovalbumin (tryptic digest) Protein Tryptic digest of BSA Water

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Component 1 Component 10 Component 104 Component 11 Component 114 Component 115 Component 117 Component 12 Component 13 Component 137 Component 14 Component 15 Component 16 Component 160 Component 162 Component 17 Component 177 Component 18 Component 183 Component 19 Component 2 Component 20 Component 21 Component 22 Component 23 Component 230 Component 24 Component 244 Component 25 Component 26 Component 263 Component 27 Component 28 Component 29 Component 3 Component 30 Component 31 Component 32 Component 33 Component 34 Component 35 Component 36 Component 37 Component 38 Component 39 Component 4 Component 40 Component 41 Component 42 Component 43 Component 44 Component 45 Component 46 Component 47 Component 48 Component 49 Component 5 Component 50 Component 52 Component 53 Component 54 Component 55 Component 57 Component 58 Component 59 Component 6 Component 60 Component 61 Component 64 Component 69 Component 7 Component 70 Component 71 Component 72 Component 74 Component 75 Component 8 Component 82 Component 83 Component 9 Ipilimumab Peptide 1 Peptide 10 Peptide 11 Peptide 12 Peptide 13 Peptide 14 Peptide 15 Peptide 16 Peptide 17 Peptide 18 Peptide 19 Peptide 2 Peptide 20 Peptide 21 Peptide 22 Peptide 23 Peptide 24 Peptide 8 Reference Peak

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CAS Number

477202-00-9

Class

Biotherapeutic glycopeptide Oligosaccharide Peptide Protein Recombinant Human IgG1κ mAb Trypsin digested

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Manufacturer

Thermo Fisher Scientific Thermo Scientific

Model

Acclaim 120 C18 Acclaim 120 C18 RSLC Acclaim 300 C18 Acclaim RSLC 120 C18 Acclaim RSLC C18 Acclaim VANQUISH C18 Accucore Vanquish C18+ Analytical CarboPac PA200 CarboPac PA200 Guard Hypersil GOLD Thermo Scientific Accucore Amide HILIC

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Amide C18 C18 Selectivity Quaternary ammonium ion Silica C18

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Displaying 1-5 of 5 results for: peptide mapping

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fast reproducible peptide mapping an pertuzumab igg1 by using a smart digest kit fully porous 120 a c18 column

Fast and Reproducible peptide mapping of an Pertuzumab (IgG1) by using a SMART digest Kit and fully porous 120 A C18 column.

Instrument Type: UHPLC

The Thermo Scientific Dionex Ultimate 3000 system is applied for the peptide mapping analysis of an Pertuzumab (IgG1). The separation was performed on a Thermo Scientific Acclaim RSLC 120 C18 column by using a Thermo Scientific SMART digest kit at UV detection of 214 nm. The SMART Digest kit provides significant improvements in reproducibility, which results in fewer sample failures, higher throughput and the ability to more easily interrogate data..

fast reproducible peptide mapping an peptibody romiplostim by using a smart digest kit solid core c18 column

Fast and Reproducible peptide mapping of an peptibody (romiplostim) by using a SMART digest Kit and solid core C18 column

Instrument Type: UHPLC

The Thermo Scientific Dionex Ultimate 3000 system is applied for the peptide mapping analysis of an peptibody (Romiplostim). The separation was performed on a Thermo Scientific Vanquish C18+ column by using a Thermo Scientific SMART digest kit at UV detection of 210 nm. The SMART Digest kit provides significant improvements in reproducibility, which results in fewer sample failures, higher throughput and the ability to more easily interrogate data.

longterm stable peptide mapping using vanquish uhplcsystem

Long-term stable peptide mapping using the Vanquish UHPLCsystem

Instrument Type: UHPLC

Peptide mapping is one of the major analyses in biotherapeutics characterization. Here we show peptide mapping with the Thermo Scientific Vanquish UHPLC system. An efficient and reliable separation of peptides obtained from a bovine serum albumin (BSA) digest is done on a Thermo Scientific Acclaim RSLC 120, C18, 2.2 µm Analytical column. Excellent retention time and area precisions as well as peak capacities were achieved over a time period of more than 80 injections.

reliable results peptide mapping using vanquish flex uhplc system

Reliable Results in Peptide Mapping Using the Vanquish Flex UHPLC System

Instrument Type: UHPLC

Peptide mapping is one of the major analyses in biotherapeutics characterization. Here we show peptide mapping with the Thermo Scientific Vanquish Flex UHPLC system. An efficient and reliable separation of peptides obtained from a bovine serum albumin (BSA) digest is done on an Thermos Scientific Acclaim RSLC 120, C18, 2.2 µm Analytical column. Excellent retention time and area precisions allow reliable qualitative and quantitative data interpretation.

column temperature control peptide mapping

Column Temperature Control in Peptide Mapping

Instrument Type: UHPLC

The Thermo Scientific Vanquish UHPLC system was used in combination with the Thermo Scientific Acclaim RSLC 120 C18, 2.2 µm, 2.1x250 mm column to provide high resolution peptide separation of a monoclonal antibody digest. The column was thermostatted at 40, 60, and 80 °C. Both still-air and forced-air mode were used. The highest peak capacity was obtained at 80 °C, and with still-air thermostatting mode. At 40 °C, the peak capacity obtained with still-air and forced-air was comparable.

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