An HPAE-PAD method was developed to determine three sugar substitutes (erythritol, allulose, and sucralose) in sugar-free foods and beverages. These sugar substitutes are often used in combination in food and beverages to create the same level of sweetness as natural sugars as well as minimize less desired flavors of these sugar substitutes. The separation of the sugars was achieved on Dionex™ CarboPac™ PA20 column. We analyzed five commercial sugar-free products for their erythritol, allulose, and sucralose contents. In addition we demonstrate this application using a PdH reference electrode.
本方法采用 Accucore C18, 2.6µm 4.6*150 mm，20%甲醇/水作为流动相，对三氯蔗糖进行了方法学考察，证明该方法完全可以满足并优于 GB 22255-2014 方法的灵敏度要求。 本文考察了甲醇和乙腈作为流动相体系，在RI检测过程中对系统噪音的影响，结果表明，同等条件下，甲醇流动相对示差检测器的背景噪音贡献，明显低于乙腈流动相；通过综合对比，确定本方法采用20%甲醇/水作为流动相。由于三氯蔗糖检测采用的是等度淋洗，经色谱柱流出的成分也会贡献RI检测器的噪音，因此色谱柱选择时，建议选择冲洗干净、柱流失小的色谱柱，如Accucore C18色谱柱。
Contaminants of emerging concern (CEC) in the environment are generally described as compounds that are unknown or unrecognized, undetected or not routinely monitored, and represent a diverse group of chemicals that may pose a risk to human health and the environment. Due to limited analytical capability and available resources , monitoring of CECs has been focused on selected analytes rather than a holistic approach. We describe a new analytical method that can be used in the quantitative analysis of 61 targeted and 312 non-targeted CECs.
Acesulfame potassium, saccharin, aspartame, and sucralose are all commonly used artificial sweeteners that have been approved for use by the United States Food and Drug Administration (FDA). Sodium cyclamate has been banned in the United States, although it is approved for use in many other countries, including the UK. We demonstrate the use of the Thermo Scientific Accucore RP-MS HPLC column for the rapid analysis of five artificial sweeteners.
We develop an HRAM-based LC-MS/MS workflow to quantitatively analyze contaminants of emerging concern (CECs) in biosolids samples and to screen for 381 non-targeted CECs. Unlike LC-QQQ-MS/MS, the Fisher Scientific Exactive Plus Orbitrap full-scan high-resolution, accurate-mass (HRAM) mass spectrometry provides not only quantitative data for targeted compounds but also information on the non-targeted compounds, such as environmental transformation by-products, for possible environmental loadings and ecological effects that would not be available in a targeted assay.
