To demonstrate practical approaches that can be used to significantly improve throughput of the cephradine USP assay monograph keeping to the spirit of USP-NF Chapter <621> guidelines while maintaining USP quality acceptance criteria.
The Thermo Scientific UltiMate 3000 HPLC system is applied for the determination of cephalosporins. The separation is performed on a Thermo Scientific Syncronis C18 HPLC column, with UV detection at 254 nm.
The Thermo Scientific HPLC system is applied for the analysis of antibiotics. The separation is performed on a Thermo Scientific Hypersil GOLD HPLC column, with UV detection at 254 nm.
