The Thermo Scientific Vanquish Flex UHPLC system is applied for the chromatographic purity analysis of Cefpodoxime Proxetil. The results obtained for the resolution between the peaks due to S-epimer and R-epimer was 4.5 (USP criteria of minimum 4.0) and theoretical plates for R-epimer were 25,623 (USP criteria of minimum 19,000). By modernizing an older USP monograph after the regulation provided in USP General Chapter <621>, the run time can be reduced by 64% and solvent consumption can be lowered by almost 74% by simply migrating to a 3µm column.
The Thermo Scientific Vanquish Flex Binary UHPLC system is applied for the related substances analysis of Telmisartan and Amlodipine Besylate tablets. The analysis was performed on a Thermo Scientific Acclaim RSLC 120 C18 column. The separation results and peak asymmetry were satisfactory.
The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of the related substances of sumatriptan succinate. The analysis was performed on a Thermo Scientific Acclaim Mixed-Mode WCX-1 HPLC column. The separation results and peak asymmetry were satisfactory.
The Thermo Scientific Dionex UltiMate 3000 LC Bio-LC system equipped with a column pre-heater is applied for the Reverse phase analysis of Romiplostim. The separation was performed on a Thermo Scientific MAbPac RP column with UV detection at 280 nm.
The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the composition and related substances analysis of Colistin Sulfate. The analysis was performed on a Thermo Scientific Acclaim 120 C18 HPLC column using the method described in the EP-8.4 monograph. The results obtained for peak resolution met the criteria stated in the EP.
