The Thermo Scientific Acclaim Surfactant Plus column and charged aerosol detection are powerfully combined in the Thermo Scientific Vanquish UHPLC system to quickly characterize a complex mixture of chelants, ionic and non-ionic surfactants from a multipurpose alkaline cleaning concentrate. Because the charged aerosol detector can quantify any non-volatine analyte separated by HPLC, this method could be made quantitative for use in cleaning validation studies.
The Thermo Scientific Acclaim Surfactant column is used for the analysis of Triton X-100, with ESI-MS detection. Triton X-100 is one of the most commonly used nonionic detergents.
The Thermo Scientific Acclaim Surfactant Plus column can provide a detailed profile of the variation in ethoxylation number of the alkylphenolethoxylate family of surfactants. In this work a simple isocratic method is used to provide a detailed profile of Triton X-100 which is a prime example of this type of surfactant.
The Thermo Scientific UltiMate 3000 RSLC system is applied for the analysis of cationic, nonionic, amphoteric and anionic surfactants. The separation is performed using a Thermo Scientific Acclaim Surfactant Plus column, with charged aerosol detection. The Thermo Scientific Acclaim Surfactant Plus column, based on novel mixed mode chromatography technology and advanced surface chemistry, provides both reversed-phase and anion-exchange retention mechanisms.
