A demonstration of the implications of salt formation on uniform response when using charged aerosol detection and a ternary inverse gradient.
To demonstrate the advantages of using the Thermo Scientific™ Hypersil GOLD™ VANQUISH, 1.9 µm column and Thermo Scientific™ Vanquish™ UHPLC system, for the fast analysis of 13 antiretroviral compounds.
The antiretroviral drug tenofovir is in formulation given as the prodrug tenofovir disoproxil fumarate (TDF) in combination with the nucleoside reverse-transcriptase inhibitor emtricitabine. Here we show the impurity profiling of an active pharmaceutical ingredient with the Thermo Scientfic Vanquish Flex UHPLC system. The simultaneous separation of early-eluting polar compounds and later-eluting nonpolar compounds is achieved by applying a ternary gradient with the quaternary Vanquish F pump using a Thermo Scientific Accucore aQ column.
The Thermo Scientific HPLC system is applied for the analysis of Tenofovir from human plasma. The sample was prepared using SOLA SCX solid phase extraction (SPE), and the separation performed on a Thermo Scientific Hypersil GOLD HPLC column in less than 3 minutes, with MS/MS detection. For Research Use Only. Not for use in diagnostic procedures.
