Sensitivity of steviol glycoside determination by HILIC UHPLC was found to increase by up to 40% by utilizing the Thermo Scientific LightPipe Diode Array Detector (DAD) Flow Cell technology. Compared to the Thermo Scientific UltiMate 3000 system, the %RSD of peak width at half height was found to be up to 80% lower in the case of dulcoside A using the Thermo Scientific Vanquish Flex system’s improved proprietary pump technology. The unique chemistry of the Thermo Scientific AcclaimTrinity P1 columns allows for the HILIC separation of highly polar and structurally similar compounds
This application combines the Acclaim Trinity P1 with charged aerosol and diode array detection to quantify stevioside sweeteners used in foods and beverages. The two detectors are complimentary at higher concentrations of steviosides, but under the conditions used here the charged aerosol detector provides superior sensitivity for most of the analytes.
