Thermo Scientific AppsLab Library: Sensitive HILIC UHPLC-UV determination of steviol glycoside natural sweeteners

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Sensitive HILIC UHPLC-UV determination of steviol glycoside natural sweeteners Be the first to rate this application

#	Compound Name	Compound Class	RT (min)
1	Dulcoside A	Steviol	3.64
2	Stevioside	Steviol	3.80
3	Rebaudioside C	steviol	4.50
4	Rebaudioside A	Steviol	4.90
5	Steviolbioside	Steviol	5.50
6	Rebaudioside B	Steviol	6.89

Description

Sensitivity of steviol glycoside determination by HILIC UHPLC was found to increase by up to 40% by utilizing the Thermo Scientific LightPipe Diode Array Detector (DAD) Flow Cell technology. Compared to the Thermo Scientific UltiMate 3000 system, the %RSD of peak width at half height was found to be up to 80% lower in the case of dulcoside A using the Thermo Scientific Vanquish Flex system’s improved proprietary pump technology. The unique chemistry of the Thermo Scientific AcclaimTrinity P1 columns allows for the HILIC separation of highly polar and structurally similar compounds

Market:	Food and Beverage
Keywords:	HILIC, UltiMate 3000, UV, UHPLC, Food Safety, QA/QC, Environment, food and beverage analysis, Vanquish Flex Quaternary, DAD Flow Cell LightPipe, natural sweeteners, Acclaim Trinity P1, steviol glycosides
Matrix:	Water
Author:	Aaron Lamb, Joanne Jones
Affiliation:	Thermo Fisher Scientific, Runcorn, UK

Uploaded on 10/27/2017.

For Research Use Only. Not for use in diagnostic procedures.