The Thermo Scientific™ Vanquish™ Duo for Inverse Gradient is used for a multidetector analysis of extractables from plastic food contact and pharmaceutical products. A Thermo Scientific Hypersil GOLD 1.9 µm column was used with charged aerosol, MS and UV detection at 230 nm. The 35 minute method allows quantification of unknown nonvolatile contaminants and clearly classifies unknowns as semivolatiles or nonvolatiles. The inverse gradient is implemented without baseline drift or detrimental impact on MS LOQs and a capillary mixer improves quantification accuracy.
This application demonstrates reliable verification and quantification of the presence of extraneous compounds in a sample, such as impurities, degradation products or extractables and leachables.
This application highlights the complimentary sensitivity of UHPLC with charged aerosol and diode array detection for determination of hoodigosides in plant extracts and dietary supplements. Eight oxypregnane steroidal glycosides isolated from dried plant material are separated from one another and from excipients such as stearic acid within 20 min on a Thermo Scientific™ Vanquish ™ UHPLC system paired with a Thermo Scientific™ Vanquish™ C18 analytical column and detected by both charged aerosol and UV/Vis diode array detectors.
Free fatty acids by gradient HPLC and Corona Veo charged aerosol detection
Five fatty acids are analyzed using a modification of the reversed-phase Universal Lipids method conditions on an Thermo Scientific™ Accucore™ C18 column. Like many lipids, free fatty acids do not have a chromophore, indicating the use of a universal detector, such as the Thermo Scientific™ Dionex™ Corona™ charged aerosol detector. The five acids shown here with their limits of quantitation in nanograms on column are: lauric acid (190 ng o.c.), myristic acid (10 ng o.c.), palmitic acid (5 ng o.c.), oleic acid (3 ng o.c.), and stearic acid (1 ng o.c.). This gradient method can be adjusted to accommodate different matrices and lipid content.
