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Fatty Acids by Universal RP-HPLC-CAD Be the first to rate this application

Description

Five fatty acids are analyzed using a modification of the reversed-phase Universal Lipids method conditions on an Thermo Scientific™ Accucore™ C18 column. Like many lipids, free fatty acids do not have a chromophore, indicating the use of a universal detector, such as the Thermo Scientific™ Dionex™ Corona™ charged aerosol detector. The five acids shown here with their limits of quantitation in nanograms on column are: lauric acid (190 ng o.c.), myristic acid (10 ng o.c.), palmitic acid (5 ng o.c.), oleic acid (3 ng o.c.), and stearic acid (1 ng o.c.). This gradient method can be adjusted to accommodate different matrices and lipid content.
Market: Food and Beverage
Matrix: Methanol / chloroform (1:1)
Uploaded on 7/12/2013.

For Research Use Only. Not for use in diagnostic procedures.