The Thermo Scientific™ Vanquish™ Duo for Inverse Gradient is used for a multidetector analysis of extractables from plastic food contact and pharmaceutical products. A Thermo Scientific Hypersil GOLD 1.9 µm column was used with charged aerosol, MS and UV detection at 230 nm. The 35 minute method allows quantification of unknown nonvolatile contaminants and clearly classifies unknowns as semivolatiles or nonvolatiles. The inverse gradient is implemented without baseline drift or detrimental impact on MS LOQs and a capillary mixer improves quantification accuracy.
This application demonstrates reliable verification and quantification of the presence of extraneous compounds in a sample, such as impurities, degradation products or extractables and leachables.
Free fatty acids by gradient HPLC and Corona Veo charged aerosol detection
Five fatty acids are analyzed using a modification of the reversed-phase Universal Lipids method conditions on an Thermo Scientific™ Accucore™ C18 column. Like many lipids, free fatty acids do not have a chromophore, indicating the use of a universal detector, such as the Thermo Scientific™ Dionex™ Corona™ charged aerosol detector. The five acids shown here with their limits of quantitation in nanograms on column are: lauric acid (190 ng o.c.), myristic acid (10 ng o.c.), palmitic acid (5 ng o.c.), oleic acid (3 ng o.c.), and stearic acid (1 ng o.c.). This gradient method can be adjusted to accommodate different matrices and lipid content.
