The Thermo Scientific™ Vanquish™ Flex UHPLC system is applied for the Related substance analysis of Caspofungin. The analysis was performed on the Thermofisher Scientific Acclaim C30 HPLC Column. The results obtained for the Resolution and theoretical plates met the criteria.
This application note is intended to demonstrate the usability of Vanquish Core instruments for the straightforward application of compendial methods.
Guidance on generating an intelligent, automated workflow for batch-release analysis of an active pharmaceutical ingredient.
Like the majority of pharmacopeial LC methods, the chromatographic analysis of temozolomide and its four related impurities given by European and United States Pharmacopeia relies on reversed-phase separation. However, the analytes are very hydrophilic, and the method is based on highly aqueous mobile phase and ion-pairing reagents. Thus, hydrophilic interaction liquid chromatography (HILIC) appears as a convenient alternative. In the current work, a new HILIC method was set up and was successfully transferred from an Agilent 1260 Infinity LC (1260 Infinity) system to a Vanquish Core HPLC.
