To demonstrate the application of a software-based method development approach for the advanced separation of all known water-soluble vitamins in reversed-phase mode with ultraviolet detection. To achieve transferability of the predicted method with excellent agreement of predicted and experimental retention times.
To describe a workflow for the simultaneous quantitative analysis of fatsoluble and water-soluble vitamins in drinks and food supplement tablets using the Thermo Scientific™ Vanquish™ Flex Duo UHPLC system and the Thermo Scientific™ Acclaim™ Polar Advantage II column.
• To demonstrate the capability of the Thermo Scientific™ Acclaim™ PolarAdvantage II (PA2) and Thermo Scientific™ Vanquish™ Flex Binary UHPLC system combination for the rapid separation of water soluble vitamins in sports beverages with excellent linearity, reproducibility, resolution, and recoveries. • To show the capability of the Vanquish Flex Binary UHPLC system to provide superior retention time precision through its novel pump features.
