To demonstrate the application of a software-based method development approach for the advanced separation of all known water-soluble vitamins in reversed-phase mode with ultraviolet detection. To achieve transferability of the predicted method with excellent agreement of predicted and experimental retention times.
The Thermo Scientific HPLC system is applied for the determination of water-soluble vitamins in vitamin tablets. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage (PA) HPLC column, with UV detection at various wavelengths. Some vitamins are strongly hydrophilic, so the column needs to operate in 100% aqueous buffer to gain sufficient retention to resolve them from the matrix. The Acclaim PA can do this reliably whereas a hydrophobic C18 column would likely suffer dewetting.
