The method transfer between the Thermo Scientific™ UltiMate™ 3000 UHPLC system and a Thermo Scientific™ Vanquish™ Flex UHPLC system is seamless and, in this case, for an isocratic separation requires no method redevelopment. The Thermo Scientific™ Acclaim™ Mixed Mode WAX-1 column proved capable of the analysis of neutral and ionic compounds that would be otherwise poorly retained on solely reversed-phase columns.The %RSD of peak area was found to be at least 50% lower for the majority of compounds analysed using the Thermo Scientific Vanquish Flex systems proprietary autosampler technology.
The analysis of food additives in carbonated beverages is important as many additives are controlled as part of the formulations of these beverages. In addition, analysis may detect possible counterfeiting of branded products. Being able to identify and quantify food additives in beverages quickly and with high sensitivity is therefore important.
Acesulfame potassium, saccharin, aspartame, and sucralose are all commonly used artificial sweeteners that have been approved for use by the United States Food and Drug Administration (FDA). Sodium cyclamate has been banned in the United States, although it is approved for use in many other countries, including the UK. We demonstrate the use of the Thermo Scientific Accucore RP-MS HPLC column for the rapid analysis of five artificial sweeteners.
The Thermo Scientific HPLC system is applied for the analysis of soft drink ingredients. The separation is performed using a Thermo Scientific Acclaim Organic Acid (OA) column, with UV detection at 210 nm. The Thermo Scientific Acclaim OA column is uniquely suited because of its aqueous compatibility for the separation of organic acids, and its hydrophobic selectivity for the separation of preservatives.
Three formats of the Thermo Scientific Acclaim 120 C18 column are used for the analysis of aspartame and its impurities, with UV detection at 254nm.
