HPLC-MS/MS separation for the tobacco alkaloids nicotine, nornicotine, cotinine, norcotinine, trans-3’-hydroxycotinine, and anabasine.
A Thermo Scientific HPLC system is applied with the Thermo Scientific Syncronis aQ HPLC column for the analysis of alkaloids in less than three minutes with UV detection at 254 nm.
The Thermo Scientific HPLC system is applied for the analysis of alkaloids. The separation is performed on a Thermo Scientific Hypersil GOLD aQ HPLC column, with UV detection at 260 nm.