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Rapid sample preparation and peptide mapping analysis of top-selling monoclonal antibody therapeutics Be the first to rate this application

peptide mapping mabs


The Thermo Scientific SMART Digest Kit is applied for generation of peptides from therapeutic monoclonal antibodies in 60 minutes. Peptide mapping samples were analysed on a Thermo Scientific UltiMate U3000 RSLC system coupled to a Thermo Scientific Q Exactive HF with Biopharma Option. Separation of sample peptides was performed on a Thermo Scientific Acclaim RSLC 120, C18, 2.2 µm analytical column using a 30 minute separation gradient, yielding excellent chromatographic separation and high sequence coverage.
Market: BioPharma
Keywords: LC-MS/MS, Monoclonal antibody, Peptide mapping, mAb, SMART Digest kit, Cetuximab, Bottom up analysis, Primary sequence, NIBRT, Bioprocessing Research
Matrix: Digest
Author: Amy Farrell, Jenny Ho, Jonathan Bones and Ken Cook
Affiliation: NIBRT, Dublin, Ireland
Uploaded on 12/22/2016.

For Research Use Only. Not for use in diagnostic procedures.