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Assay and related substances analysis of Cefotaxime sodium using a C18 HPLC column as per JP XVI and KP X monograph methods Be the first to rate this application

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0102030405060Time [min]-50050100150200250Response [mAU]
# Compound Name Compound Class RT (min)
1 Desacetyl Cefotaxime Impurity 3.41
2 Cefotaxime beta-lactam antibiotic 11.92

Description

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the assay and related substances analysis of Cefotaxime Sodium. The analysis was performed on a Thermo Scientific Syncronis aQ HPLC column using the method described in the JP XVI/KP X. The results obtained for resolution and symmetry factor met the criteria stated in the JP/KP. The results obtained for resolution between the peaks due to desacetyl Cefotaxime and Cefotaxime was 32.8(JP/KP criteria of minimum 20.0) and symmetry factor for cefotaxime was 1.0 (JP/KP criteria of maximum 2).
Market: Pharma
Keywords: Cefotaxime, HPLC, Syncronis aQ, UV detection, JP XVI monograph, KP X monograph, beta-lactam antibiotic
Matrix: N/A
Author: Krishnarao Dara
Affiliation: APAC CoE Lab, Ahmedabad, India
Uploaded on 8/17/2016.

For Research Use Only. Not for use in diagnostic procedures.