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Evaluation of temperature influence on retention of glycans in HILIC Be the first to rate this application

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27121722273237Time [min]-5000000050000001000000015000000200000002500000030000000Response [counts]
# Compound Name Compound Class RT (min)
1 GU3 glycans 4.30
2 GU4 glycans 7.81
3 GU5 glycans 12.48
4 GU6 glycans 16.45
5 GU7 glycans 20.43
6 GU8 glycans 23.36
7 GU9 glycans 26.15
8 GU10 glycans 28.74
9 GU11 glycans 31.13
10 GU12 glycans 32.94
11 GU13 glycans 34.98
12 GU14 glycans 36.56

Description

The Thermo Scientific Dionex Ultimate 3000 BioRS system in combination with the Thermo Scientific Accucore 150-Amide-HILIC is used to run separation of 2-AB-labelled dextran ladder in gradient mode. The column compartment was set at different temperature between 20 and 50 °C. At high temperature the selectivity between oligomers was improved, and peaks were in average narrower. Consequently higher peak capacity was achieved at high temperature.
Market: Other
Keywords: 2-aminobenzamide, Accucore 150-Amide-HILIC, Biotherapeutics, Fluorescence detection
Matrix: 9/1 (v/v) acetonitrile/ammonium formate pH 4.5
Uploaded on 2/18/2015.

For Research Use Only. Not for use in diagnostic procedures.