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AN202: High Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) Analysis of Mannose-6-Phosphate
Description
This application note describes the determination of M-6-P in glycoproteins using HPAE-PAD. M-6-P added to hydrolyzed BSA was separated on a Thermo Scientific Dionex CarboPac PA200 analytical column using 100 mM sodium hydroxide and 100 mM sodium acetate at 0.5 mL/min for 30 min. M-1-P and M-6-P peaks were fully resolved. This application note shows that HPAE-PAD is a fast, direct method for determining the M-6-P content of a glycoprotein.| Market: | BioPharma |
| Keywords: | Electrochemical detection, phosphorylated sugars, total phosphate, phosphate, TN71, AN 202, TN 20, TN 70671, TN20, TN70671, mannose-1-phosphate, Mannose-6-phosphate |
| Matrix: | BSA |
| Author: | Terri Christison and Jeffrey Rohrer |
| Affiliation: | Thermo Fisher Scientific, Sunnyvale, CA USA |
For Research Use Only. Not for use in diagnostic procedures.
