AN202: High Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) Analysis of Mannose-6-Phosphate

Instrument Type: IC

This application note describes the determination of M-6-P in glycoproteins using HPAE-PAD. M-6-P added to hydrolyzed BSA was separated on a Thermo Scientific Dionex CarboPac PA200 analytical column using 100 mM sodium hydroxide and 100 mM sodium acetate at 0.5 mL/min for 30 min. M-1-P and M-6-P peaks were fully resolved. This application note shows that HPAE-PAD is a fast, direct method for determining the M-6-P content of a glycoprotein.