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Separation of Related Impurities from Vercuronium Bromide as per BP monograph.

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of Vercuronium Bromide. The separation was performed on a Thermo Scientific Acclaim Polar Advantage-II HPLC column using the method described in BP monograph. The results obtained for Resolution exceeded the criteria stated in the BP. Resolution between Vercuronium Bromide and Impurity-C was 8.97 ( met BP criteria of NLT 1.2), Resolution between Vercuronium Bromide and Impurity-F was 2.15 (met BP criteria of NLT 1.2) and Resolution between Vercuronium Bromide and Impurity-B was 10.78 (met BP criteria NLT 2.0).