Evaluation of Prelude SPLC and TSQ Endura Mass Spectrometer in Research Analysis of Testosterone in Human Serum

Instrument Type: LCMSMS

The analysis of testosterone in human serum for clinical research is currently performed using many different sample preparation procedures. In this note, we evaluated a Prelude SPLC system coupled to a TSQ Endura MS (Figure 1) in the analysis of testosterone using two sample preparation methods: online sample cleanup and offline sample cleanup. Each method uses specific chromatography, but both share the same mass spectrometer settings and parameters.