AU178: A Faster Solution with Increased Resolution for Determining Chromatographic Identity and Absence of OSCS in Heparin Sodium

Instrument Type: IC

Here, an improved method for resolving DS and OSCS in heparin with an IonPac AS11-HC (2 × 250 mm) column is demonstrated, which is similar to the US FDA method previously described. AU178 updates Application Note 235. The higher capacity of the IonPac AS11-HC column, relative to the IonPac AS11 column described in the current USP method, minimizes the possibility of column overload and provides improved resolution between DS and heparin. The microbore column format reduces eluent consumption and hence the time and labor required for manual eluent preparation.