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USP 38 monograph: impurity determination of caffeine using a C18 HPLC column
Instrument Type: HPLCThe Thermo Scientific Dionex UltiMate 3000 LC system is applied for the analysis of Caffeine and its impurities. The separation was performed on a Thermo Scientific Acclaim 120 C18 HPLC column using the method described in the USP 38 monograph. The results obtained for resolution and tailing factor exceeded the criteria stated in the USP. Resolution between theophylline and caffeine was 12.3 (USP criteria of not less than 6.0) and tailing factor for theophylline and caffeine was 1.1 (USP criteria not more than 2.0).
Improved analysis of xanthines using a Thermo Scientific Hypersil GOLD aQ HPLC column
Instrument Type: HPLCThe Thermo Scientific HPLC system is applied for the analysis of xanthines. The separation is performed on a Thermo Scientific Hypersil GOLD aQ HPLC column, with UV detection at 254 nm.
