Quantitative Measurement of Plasma Free Metanephrines by Ion-Pairing Solid Phase Extraction and LC-MS/MS with Porous Graphitic Carbon Column

Instrument Type: LCMSMS

Plasma free metanephrine (MN) and normetanephrine (NMN), collectively known as Pmets, are important molecules for clinical research. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become widely used to measure Pmets because of its high analytical sensitivity and specificity. Because Pmets are very polar, special solid phase extraction (SPE) and chromatographic methods have been developed for their analysis. Ion-paring (IP)-SPE, which has been used to purify a wide range of polar compounds, is well suited for the purification of Pmets.

Plasma Free Metanephrines Quantitation with Automated Online Sample Preparation and Liquid Chromatography-Tandem Mass Spectrometry

Instrument Type: LCMSMS

Plasma free metanephrine (MN) and normetanephrine (NMN), collectively known as Pmets, are preferred biomarkers for pheochromocytoma for clinical research. LC-MS/MS has become widely used to measure Pmets because of its high analytical specificity. Although our recently reported method utilizing LC-MS/MS using IP-SPE and PGC column chromatography is fast and analytically sensitive, it can be further improved by automating the offline sample preparation with online sample preparation technology, which is more time- and cost-effective.