Essential Lipidomics Experiments Using the LTQ Orbitrap Hybrid Mass Spectrometer

Instrument Type: LCMSMS

We tested the performance of a novel mass spectrometer, the Thermo Scientific LTQ Orbitrap, in a multitude of different applications in the field of lipidomics. The experiments presented here take advantage of the LTQ Orbitrap capabilities of multiple stage fragmentation, high resolution and mass accuracy to solve challenges of quantitating isomeric glycerophospholipids, resolving isobaric species, and elucidating fragmentation pathways and compositional assignment of various lipid species.

Structural Determination of Flavonoids Using MSn

Instrument Type: LCMSMS

Flavonoids, a class of polyphenolic compounds found in tea, plants and many fruits, have shown some promising results related to anti-cancer as well as anti-inflammatory and anti-allergy properties. For these reasons, there has been renewed interest in both detecting and characterizing these compounds. We demonstrate the utility of MSn for structural determination and unambiguous classification of a suspected flavonoid compound and unambiguous determination of product origin.