Quantitative Analysis of Ractopamine in Beef using Automated Online Sample Preparation with Liquid Chromatography-Tandem Mass Spectrometry

Instrument Type: LCMSMS

Ractopamine is a phenethanolamine member of the family of b2-adrenergic receptor agonists (b-agonists). It has been widely used as a veterinary additive drug in livestock production to promote leanness in meat, accelerate average daily weight gain and improve feed efficiency. Recently, there have been growing concerns about the safety of meat containing ractopamine residues due to its potential health risks for humans. We describe a rapid and sensitive automated online sample preparation LC-MS/MS method to determine ractopamine in beef.

Fast analysis of β-agonists using a Thermo Scientific Accucore Phenyl-Hexyl HPLC column

Instrument Type: LCMS

The Thermo Scientific HPLC system is applied for the analysis of β-agonists. The separation is performed on a Thermo Scientific Accucore Phenyl-Hexyl column, with MS detection.

Effective extraction of beta-agonists from pork using in-line SPE

Instrument Type: HPLC

In this example, pork was extracted with organic solvents in a Thermo Scientific ASE Accelerated Solvent Extractor. Evaporation and reconstitution were difficult, so it was overcame by using solid-phase extraction (SPE). SPE is most effective when it is orthogonal to the analytical separation, so a Thermo Scientific Acclaim Mixed-Mode WCX-1 cartridge was used to retain the drugs, and washed away lipids and other interferences with organic solvent. The auxiliary valve then transferred the drugs to an Acclaim C30 column for RP-HPLC analysis.

Rapid and sensitive determination of clenbuterol, ractopamine and phenolethanolamine in meat using a Thermo Scientific Acclaim PolarAdvantage HPLC column

Instrument Type: HPLC

The Thermo Scientific UltiMate 3000 RSLC system is applied for the determination of clenbuterol, ractopamine and phenolethanolamine in meat. The separation is performed on a Thermo Scientific Acclaim PolarAdvantage HPLC column after automated on-line solid phase extraction using a Thermo Scientific SolEX HRP cartridge, with UV detection at 210 nm.