AN82: Analysis of Fruit Juices Adulterated with Medium Invert Sugar from Beets (Method B).

Instrument Type: IC

Fruit juice adulteration presents an economic & regulatory problem. Investigators using high performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) discovered several components in beet medium invert sugar(BMIS) that are not present in orange juice. The selectivity of anion-exchange chromatography, especially for oligosaccharides, the sensitivity and specificity of pulsed amperometric detection make HPAE-PAD uniquely suited to this analysis. Today we would use a different electrochemical waveform (see TN21) and post-column addition of NaOH would not be required

AN159: Determination of Sucralose Using HPAE-PAD

Instrument Type: HPLC (Biocompatible)

This application note describes the use of HPAE- PAD to determine sucralose in food and beverage samples. Red Raspberry Diet Rite is a soft drink artificially sweetened with sucralose. It also contains natural and artificial flavors, organic compounds (potassium benzoate and citrate), food dye, and another nonnutritive sweetener (acesulfame potassium). These model food and beverage samples present a great challenge for most chromatographic methods. In this note, the CarboPac™ PA20 anion-exchange column is used to separate sucralose from other ingredients in Red Raspberry Diet Rite and Splenda.