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High resolution separation of cyclophosphamide from polyethylene glycol (PEG) in formulation using a reversed-phase HPLC-UV method

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 system is applied for the determination of cyclophosphamide in formulation. The high resolution separation of cyclophosphamide from PEG was performed on a Thermo Scientific Syncronis C18 HPLC column, with a run time of 20 minutes.