Simplified, high-throughput separation of glucocorticoids

Instrument Type: UHPLC

Improved separation of closely related glucocorticoids with reduced method complexity High-throughput analysis possible through a reduced complexity, rapid, two-minute isocratic method Associated reduction in cost per sample through reduced mobile phase consumption and waste generation

Rapid determination of prednisone using a Thermo Scientific Accucore C18 HPLC column and MS detection

Instrument Type: LCMSMS

A simple, rapid and sensitive procedure for the determination of prednisone by liquid chromatography-tandem mass spectrometry was developed and evaluated. The drug was separated on an Accucore C18 HPLC column under reversed-phase, gradient conditions. Detection was performed on a triple quadrupole mass spectrometer using positive polarity, heated electrospray ionisation (HESI) conditions operating in selected reaction monitoring (SRM) mode.