AN1070: Determination of Inositol Phosphates in Dried Distillers Grains with Solubles

Instrument Type: IC

A method to determine the level of phytate hydrolysis products in dried distiller grains with soluble (DDGS) is useful in determining phosphorus bioavailability to livestock animals. This study demonstrates the determination of 25 inositol phosphate isomers—InsP2–InsP6—in an in-house prepared reference solution with the method applied to a DDGS sample. Separation of the InsP isomers was achieved using a Dionex CarboPac PA100 column (4 × 250 mm) in <42 min with an HCl gradient, followed by a postcolumn reaction with ferric nitrate in dilute perchloric acid and then UV detection.

AN295: Determination of Phytic Acid in Soybeans and Black Sesame Seeds

Instrument Type: IC

Phytic acid is the storage form of phosphate in many plant tissues, especially seeds and grains. Because phytic acid is a good metal chelator, it is believed to have a negative nutritional impact on strongly chelating metals necessary for good health. The method reported here uses the hydroxide-selective Dionex IonPac AS11 column to separate phytate from other anions (including the remaining chloride from the sample preparation) with an isocratic method.