LC-MS quantitative screening method for 18 anabolic steroids in oral fluid using MS2 spectra data collected with Q Exactive Orbitrap mass spectrometer

Instrument Type: LCMSMS

Androgenic-anabolic steroids (AAS) are drugs which mimic effects of testosterone and dihydrotestosterone in the human body. They increase protein synthesis within cells which results in buildup of cellular tissue, especially in muscles. Use of anabolic steroids by athletes to increase body weight is referred to as doping and is banned by major sporting bodies. In this work we implemented Thermo Scientific Q Exactive ultra high resolution mass spectrometer to ensure high method specificity and sensitivity.

A Sensitive and Efficient Method to Analyze THC and THCCOOH in Oral Fluid Using LC-MS/MS in Forensic Toxicology Laboratories

Instrument Type: LCMSMS

The Substance Abuse and Mental Health Services Administration (SAMHSA) lists delta9-tetrahydrocannabinol (THC) as the only target analyte for detection of cannabis use in oral fluid at 2 ng/mL confirmation cutoff concentration. In this study, we developed a sensitive and efficient method for simultaneous determination of THC and THCCOOH in OF. Both analytes were extracted using a simple and fast SPE method without pre-conditioning, evaporation, or reconstitution. THCCOOH was detected with high sensitivity (LOQ 10 pg/mL), and no derivatization procedure was required. For forensic use only.