A Sensitive and Efficient Method to Analyze THC and THCCOOH in Oral Fluid Using LC-MS/MS in Forensic Toxicology Laboratories

Instrument Type: LCMSMS

The Substance Abuse and Mental Health Services Administration (SAMHSA) lists delta9-tetrahydrocannabinol (THC) as the only target analyte for detection of cannabis use in oral fluid at 2 ng/mL confirmation cutoff concentration. In this study, we developed a sensitive and efficient method for simultaneous determination of THC and THCCOOH in OF. Both analytes were extracted using a simple and fast SPE method without pre-conditioning, evaporation, or reconstitution. THCCOOH was detected with high sensitivity (LOQ 10 pg/mL), and no derivatization procedure was required. For forensic use only.