Analysis of alkaloids in bitter orange extract by HPLC-UV

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of alkaloids in bitter orange extract which is an ingredient in traditional Chinese medicines. The separation is performed on a Thermo Scientific Acclaim 120 C18 column, with UV detection at 224 nm and is suitable for assaying ingredients and formulations for bitter orange and its active constituents. Chromatogram A is standards and chromatogram B is an extract of bitter orange.