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Displaying 1-2 of 2 results for Tag: OPA derivatization

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Fast analysis of glutamate in beer by automated precolumn OPA derivatization

Instrument Type: HPLC

The Thermo Scientific HPLC system is applied for the analysis of glutamate in beer. The sample preparation is automated, and the separation is performed using a Thermo Scientific Acclaim PolarAdvantage column, with UV detection at 330 nm. Chromatogram A is beer and chromatogram B is beer spiked with 250 µg/mL glutamic acid.

High sensitivity analysis of amino acids by automated OPA derivatization on a Thermo Scientific Acclaim PolarAdvantage (PA) HPLC column

Instrument Type: HPLC

The Thermo Scientific Dionex UltiMate 3000 system is applied for the analysis of amino acids. The amino acids are derivatized in the autosampler with OPA before separation on a Thermo Scientific Acclaim PolarAdvantage (PA) 5 µm HPLC column. The derivative provides low detection limits using either UV or fluorescence detection.