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Comparison of five reversed-phase columns for the separation of 4-chloro, 7-nitro, 1,2,3-benzoxadiazole (NBD-chloride) derivatives of volatile amines

Instrument Type: HPLC

The Thermo Scientific UltiMate 3000 HPLC system is applied for the analysis of 4-chloro, 7-nitro, 1,2,3-benzoxadiazole (NBD-chloride) derivatives of volatile amines. The separation is performed on five reversed-phase columns; Thermo Scientific Acclaim C30, Acclaim 120 C8, Acclaim 120 C18, Acclaim PolarAdvanage and Acclaim PolarAdvantage II. The results were compared.