OSHA Method 41 for improved analysis of Diethylamine using the Thermo Scientific Acclaim Mixed-Mode HILIC-1 HPLC column

Instrument Type: HPLC

As shown here, the Thermo Scientific Acclaim Mixed-Mode HILIC-1 can analyze the diethylamine derivative under the normal-phase conditions of OSHA Method 41. In this case, 4-chloro-7-nitro-1,2,3-benzoxadiazole (NBD-chloride) reacts with amines in the trap, to produce a highly-colored, fluorescent derivative. The unreacted reagent and a trace of monoethylamine eluted at 2.3 and 4.4 minutes, respectively, and neither interferes with the analyte of interest. Compared to the existing OSHA method, the new column provides improved peak shape and faster analysis time.